Dr. Aleksandar Krmpot Presents Recent COST TETRA Results During STSM Visit to Vilnius Tech
October 2025, Vilnius, Lithuania
Dr. Aleksandar Krmpot from the Institute of
Physics Belgrade (IPB) successfully completed a Short-Term Scientific Mission (STSM) to
Vilnius Tech, Lithuania, where he presented the latest research outcomes and disseminated
the objectives of COST Action CA23125 - TETRA (The mETamaterial foRmalism approach to
recognize cAncer) to the university community.
During his visit, Dr. Krmpot delivered a
comprehensive presentation titled "Nonlinear Imaging and Laser Nano Surgery of Fungal Cells
to Enable Electrophysiological Measurements," showcasing cutting-edge work from IPB. The
presentation highlighted several key achievements:
Key Research Areas Presented:
Nonlinear Optical Effects: Dr. Krmpot explained Two-Photon
Excitation Fluorescence (TPEF) techniques, demonstrating how different excitation pathways
(one-photon at 350nm versus two-photon at 700nm) produce similar blue-green fluorescence,
with TPEF offering significant advantages for biological imaging.
Label-Free Imaging Applications: The presentation showcased
their diverse capabilities, including label-free imaging of erythrocytes using ultrashort
lasers, label-free imaging of human colon tissue, and TPEF imaging of chitinous structures
in insects, demonstrating the versatility of nonlinear optical methods in biomedical
optics.
In Vivo Cell Surgery: A particular focus was placed on in vivo
two-photon imaging combined with cell wall laser nanosurgery techniques. Dr. Krmpot
demonstrated plasmolysis procedures on fungal hyphae and the subsequent TPEF imaging using
CalcoFluor White to label cell walls, enabling precise surgical patterns for
electrophysiological measurements.
The presentation emphasized an innovative
approach where fluorescence intensity increases with each scan, contrary to traditional
photo-bleaching effects.
This STSM visit strengthens the collaboration
between COST TETRA Action partners and promotes the exchange of advanced imaging techniques
that are crucial for cancer recognition and biomedical research. The Vilnius Tech community
showed great interest in the presented methodologies and their potential applications in
metamaterial-based cancer diagnostics.
About COST Action TETRA: COST Action CA23125 -
TETRA aims to develop novel approaches for cancer recognition using metamaterial formalism,
bringing together researchers from across Europe to advance diagnostic capabilities through
innovative optical and imaging techniques.
October 2025, Vilnius, Lithuania
Dr. Aleksandar Krmpot from the Institute of Physics Belgrade (IPB) successfully completed a Short-Term Scientific Mission (STSM) to Vilnius Tech, Lithuania, where he presented the latest research outcomes and disseminated the objectives of COST Action CA23125 - TETRA (The mETamaterial foRmalism approach to recognize cAncer) to the university community.
During his visit, Dr. Krmpot delivered a comprehensive presentation titled "Nonlinear Imaging and Laser Nano Surgery of Fungal Cells to Enable Electrophysiological Measurements," showcasing cutting-edge work from IPB. The presentation highlighted several key achievements:
Key Research Areas Presented:
Nonlinear Optical Effects: Dr. Krmpot explained Two-Photon Excitation Fluorescence (TPEF) techniques, demonstrating how different excitation pathways (one-photon at 350nm versus two-photon at 700nm) produce similar blue-green fluorescence, with TPEF offering significant advantages for biological imaging.
Label-Free Imaging Applications: The presentation showcased their diverse capabilities, including label-free imaging of erythrocytes using ultrashort lasers, label-free imaging of human colon tissue, and TPEF imaging of chitinous structures in insects, demonstrating the versatility of nonlinear optical methods in biomedical optics.
In Vivo Cell Surgery: A particular focus was placed on in vivo two-photon imaging combined with cell wall laser nanosurgery techniques. Dr. Krmpot demonstrated plasmolysis procedures on fungal hyphae and the subsequent TPEF imaging using CalcoFluor White to label cell walls, enabling precise surgical patterns for electrophysiological measurements.
The presentation emphasized an innovative approach where fluorescence intensity increases with each scan, contrary to traditional photo-bleaching effects.
This STSM visit strengthens the collaboration between COST TETRA Action partners and promotes the exchange of advanced imaging techniques that are crucial for cancer recognition and biomedical research. The Vilnius Tech community showed great interest in the presented methodologies and their potential applications in metamaterial-based cancer diagnostics.
About COST Action TETRA: COST Action CA23125 - TETRA aims to develop novel approaches for cancer recognition using metamaterial formalism, bringing together researchers from across Europe to advance diagnostic capabilities through innovative optical and imaging techniques.